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Set temporal interpolation to Hold as seen below:
If the only difference is bitrate, then any container which accepts variable bitrate streams, will fulfill your requirement e.g. MP4, MKV..etc Step 1 is to encode your segments, ideally using the same encoder, to different bitrates with all other parameters being the same e.g. via ffmpeg, ffmpeg -ss 0 -t 5 -i input.mp4 -b:v 1000k seg1.mp4 ffmpeg -ss 5 -t ...
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